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Asian Journal of Plant Sciences
  Year: 2010 | Volume: 9 | Issue: 6 | Page No.: 351-357
DOI: 10.3923/ajps.2010.351.357
 
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Evaluation of Cotyledonary Node of Clitoria ternatea L. for High Frequency in vitro Axillary Shoot Proliferation

J. Singh and K.N. Tiwari

Abstract:
The objective of this study was to establish mass multiplication protocol for Clitoria ternatea. An efficient protocol for in vitro propagation of the valuable medicinal and forage plant aparajita (Clitoria ternatea L.) was developed through axillary shoot proliferation. Cotyledonary node having cotyledons or devoid of cotyledons were evaluated for axillary shoot development. The effect of cytokinins, sucrose concentrations and light-dark condition on axillary shoot regeneration was investigated. Cotyledonary node with cotyledons cultured on MS medium supplemented with 1.5 mg L-1 BAP yielded the maximum number (10.8) of shoots per explant. All media supplemented with 3% sucrose and incubated under 16 h light condition found most suitable for high frequency axillary shoot proliferation. Elongated shoots produced profuse rooting after transfer to half-strength MS medium supplemented with 0.2 mg L-1 IBA. The rooted plantlets were successfully acclimatized and exhibited 95% survival after transfer into the field. This protocol is suitable for large scale production of plantlets of C. ternatea.
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How to cite this article:

J. Singh and K.N. Tiwari, 2010. Evaluation of Cotyledonary Node of Clitoria ternatea L. for High Frequency in vitro Axillary Shoot Proliferation. Asian Journal of Plant Sciences, 9: 351-357.

DOI: 10.3923/ajps.2010.351.357

URL: https://scialert.net/abstract/?doi=ajps.2010.351.357

COMMENTS
03 October, 2010
Archana upadhyay:
Clitoria ternatea is an important medicinal plan widely used in auyrvedic drugs. Its availability throughout the year is essential.Tissue culture protocol definitely fulfill the objectives.Further there is need to evaluate its pharmacological potential.
16 February, 2011
Dr KNTiwari:

Thanks for comments.I will evaluate its pharmacological potential.

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