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Assessment of Growth and Cellulase Production of Wild-Type Microfungi Isolated from Ota, Nigeria |
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S. Nwodo Chinedu,
Angela O. Eni,
Adebayo I. Adeniyi and Janet A. Ayangbemi |
Abstract:
The aim of the study was to isolate and identify filamentous microfungi involved in wood-waste decomposition in Canaanland, Ota, South-West Nigeria and to evaluate their potentials for cellulose saccharification. Microbiological techniques were used to isolate and identify the fungi. Four filamentous microfungi, identified as Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum and Trichoderma sp., were isolated. All the isolates, particularly Trichoderma sp., grew rapidly on Sabouraud’s agar and Czapek-Dox agar. Two of the isolates, Aspergillus niger and Trichoderma sp., was cultivated for 168 h by submerged fermentation in modified Czapek-Dox liquid medium containing cellulose as sole carbon source and harvested at 24 h intervals. The mycelia weight of the harvested cultures, and the protein content and cellulase activity of the filtrates were determined. The peak mycelia weight of 4.6 and 3.0 mg mL-1 was, respectively obtained for Trichoderma sp. and A. niger at 48 h. The protein and cellulase activity of Trichoderma sp. peaked at 72 h whereas for A. niger, the peak protein content and peak cellulase activity was obtained at 96 and 72 h, respectively. The peak protein and cellulase activity values of A. niger were 0.175 and 0.077 unit mL-1, respectively. Trichoderma sp. yielded a protein peak of 0.180 mg mL-1 and peak cellulase activity of 0.108 unit mL-1. There is a correlation between the protein content and cellulase activity of the culture filtrates. The strains of A. niger and Trichoderma sp. obtained from this study are potential tools for the saccharification and bioconversion of cellulosic materials.
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How to cite this article:
S. Nwodo Chinedu, Angela O. Eni, Adebayo I. Adeniyi and Janet A. Ayangbemi, 2010. Assessment of Growth and Cellulase Production of Wild-Type Microfungi Isolated from Ota, Nigeria. Asian Journal of Plant Sciences, 9: 118-125.
DOI: 10.3923/ajps.2010.118.125
URL: https://scialert.net/abstract/?doi=ajps.2010.118.125
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| COMMENTS |
23 December, 2014
christian :
please I need an extract of Trichoderma reesei |
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25 December, 2014
S.N. Chineedu:
Unfortunately, we do not any strain of Trichoderma sp. in the lab presently.
Thanks. |
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