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Asian Journal of Plant Sciences

Year: 2008 | Volume: 7 | Issue: 3 | Page No.: 327-330
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Authors

F.A. Gutiarrez-Miceli

T. Ayora-Talavera

M. Abud-Archila

M. Salvador-Figueroa

L. Adriano-Anaya

M.L. Arias Hernandez

L. Dendooven

Keywords

The objective of this study was to investigate survival rate and growth of the orchid Guarianthe skinnerii when inoculated with or without T. harzianum during acclimatizing of the seedlings obtained after micropropagation. The G. skinnerii seedlings were obtained by in vitro germination of seeds from the capsule. Seedlings were transferred to unicel pots contained peat moss and were inoculated with T. harzianum. A treatment without inoculation served as control. The seedlings were kept in climatic chamber at 20 ± 2 ° C for 4 months (step one), in the laboratory at 25 ± 8 °C for four months (step two) and in the greenhouse at 30 ± 12 °C for four months (step three). The seedlings were distributed according to a completely aleatorized experimental design. After each step, the percentage of plants that survived, plant height, number of leaves and shoots were determined. Survival of G. skinnerii inoculated with T. harzianum was significantly 1.6 times higher than those non-inoculated seedlings. Plant height of inoculated plants was 3.6 times greater than control plants after the first step. The number of leaves was 2.5 times higher only after the step one. The amount of shoots was 1.4 times higher in the seedlings inoculated with T. harzianum. The results indicated that the survival and growth of seedlings inoculated with T. harzianum increased in extreme temperature conditions for acclimatization.
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How to cite this article

F.A. Gutiarrez-Miceli, T. Ayora-Talavera, M. Abud-Archila, M. Salvador-Figueroa, L. Adriano-Anaya, M.L. Arias Hernandez and L. Dendooven, 2008. Acclimatization of Micropropagated Orchid Guarianthe skinnerii Inoculated with Trichoderma harzianum. Asian Journal of Plant Sciences, 7: 327-330.

DOI: 10.3923/ajps.2008.327.330

URL: https://scialert.net/abstract/?doi=ajps.2008.327.330

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