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Asian Journal of Plant Sciences
  Year: 2006 | Volume: 5 | Issue: 6 | Page No.: 1002-1006
DOI: 10.3923/ajps.2006.1002.1006
 
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Random Amplified Polymorphic DNA (RAPD) study of Pistacia species (Anacardiaceae)

Mohannad G. AL-Saghir and Duncan M. Porter

Abstract:
Phylogenetic relationships among four Pistacia species were studied by randomly amplified polymorphic DNA (RAPD) analysis. The studied species were: P. khinjuk. P. lentiscus, P. terebinthus and P. vera. Genomic DNA was extracted from leaf tissue and RAPD analysis was performed using 23 primers. A total of 248 fragments were generated and 139 bands were polymorphic at the inter-specific level. Subjecting these data to phylogenetic analysis yielded a shortest cladogram that is 34 steps long comprising two main groups. P. khinjuk, P. terebinthus and P. vera, were included in one group, while P. lentiscus formed the second group. The first group included deciduous species with whereas the species included in the second group mostly grow as evergreen shrubs. The cladogram showed that the closest pairs of species were P. khinjuk and we suggest that P. khinjuk may be a descendant of P. vera.
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 RELATED ARTICLES:
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How to cite this article:

Mohannad G. AL-Saghir and Duncan M. Porter , 2006. Random Amplified Polymorphic DNA (RAPD) study of Pistacia species (Anacardiaceae). Asian Journal of Plant Sciences, 5: 1002-1006.

DOI: 10.3923/ajps.2006.1002.1006

URL: https://scialert.net/abstract/?doi=ajps.2006.1002.1006

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