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Asian Journal of Plant Pathology

Year: 2009 | Volume: 3 | Issue: 1 | Page No.: 8-13
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F. Chamandoosti

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Research Article

In vitro Selection: A Novel Source of Resistance to Sclerotinia Stem Rot in Canola (Brassica napus L.)

F. Chamandoosti
In this research, effect of different concentrations of oxalic acid (OAA) (0, 0.02, 0.2, 2 and 20 mM L-1) were studied on callusing and organogenesing of canola in order to possibility producing resistant canola plant to pathogen fungus Sclerotinia sclerotiorum (Lib.) de Bary by this pressure selection. 0-0.2 mM L-1 OAA had a positive effect on above reactions so that the mean diameter of calli and the mean number of regenerated roots and shoots on hypocotyl explants, also the mean number of explants that produced callus and de novo organs were increased. These results showed that the addition of OAA (0.0-20 mM L-1) in canola culture media (in vitro selection technique) lead to produce resistant canola plants to Sclerotinia sclerotiorum (Lib.) de Bary.
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