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American Journal of Plant Physiology
  Year: 2013 | Volume: 8 | Issue: 3 | Page No.: 114-122
DOI: 10.3923/ajpp.2013.114.122
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In vitro Antioxidant and Chemical Constituents from the Leaves of Ormocarpum cochinchinense Elumbotti

T. Sivakumar and D. Gajalakshmi

The phytochemical screening and antioxidant properties of the leaves of Ormocarpum cochinchinense L. were studied after extraction of various compounds in it using different solvents. The different solvents used for extraction was, dimethyl sulfoxiamide (DMSO), Ethyl Acetate (EtoAc), ethanol (EtOH), methanol (MeOH) and chloroform (CHCl3). Antioxidant potential was evaluated by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. The DMSO, EtoAc, EtOH, MeOH, CHCl3, extracts showed significant (p<0.001) antioxidant potentiality in a dose-dependent manner. Its IC50 values (13.05±0.39; 14.08±0.42; 15.02±0.45; 16.12±0.48; 14.88±0.44 μg mL-1) were compared to the IC50 value of the reference standard ascorbic acid (06.10±0.18; 07.18±0.21; 08.15±0.24; 09.10±0.27; 08.18±0.23 μg mL-1) which suggested that the Ormocarpum cochinchinense is a potent antioxidant. The phytochemical evaluation indicated the presence of chemical constituents including flavonoids, alkaloids, steroids, terpenoids, saponins, gums, tannins, resins, coumarins, glycosides, carbohydrates. This study also shows that the different solvent extract of leaves of O. cochinchinense has bioactivity. Further the compound needs to be isolated to confirm the activities of the individual compounds.
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How to cite this article:

T. Sivakumar and D. Gajalakshmi, 2013. In vitro Antioxidant and Chemical Constituents from the Leaves of Ormocarpum cochinchinense Elumbotti. American Journal of Plant Physiology, 8: 114-122.

DOI: 10.3923/ajpp.2013.114.122






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