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Effect of Salinity Stress on Growth, Mineral Composition, Proline Content, Antioxidant Enzymes of Soybean |
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M.R. Amirjani |
Abstract:
Present study has explored the effect of salinity stress on growth, mineral composition, proline content, antioxidant enzymes of soybean. Soil salinity is a major limitation to legume production in many areas of the world. The salinity sensitivity of soybean was studied. Soybean plants were exposed to 0, 50, 100 and 200 mM NaCl. The effect of salinity on length and fresh weight of seedling were determined. Increasing salinity level to 50, 100 and 200 mM resulted in a reduction of plant height of 30, 47 and 76% and a reduction of fresh weight of 32, 54 and 76%, respectively. The activity of nitrogenase and Ammonium content of nodules were measured by analyzing ethylene in the gas samples by chromatography and the phenol-hypochlorite method, respectively. Nitrogenase activity had a decrease of 60% and ammonium content a significant increase (100%) at 200 mM salt concentration. Proline accumulation and diamine oxidase, DAO, were studied by reading the absorption of chromophore at 520 nm using spectrophotometer. Seedlings subjected to salt stress in the presence. Both DAO activity and proline content were increased in soybean under 50 to 200 mM NaCl. Using atomic absorption spectrophotometer, ion uptake of for Na+, K+, Ca2+ and Mg2+ were determined. The Na+ content significantly increased but the contents of K+, Ca2+ and Mg2+ decreased significantly as salinity treatment concentrations increased. The behaviour of antioxidant enzymes was analyzed. A significant decrease in superoxide dismutase, catalase and peroxidase activities under 100 and 200 mM salt were found.
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How to cite this article:
M.R. Amirjani , 2010. Effect of Salinity Stress on Growth, Mineral Composition, Proline Content, Antioxidant Enzymes of Soybean. American Journal of Plant Physiology, 5: 350-360.
DOI: 10.3923/ajpp.2010.350.360
URL: https://scialert.net/abstract/?doi=ajpp.2010.350.360
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25 February, 2015
Norov Toxir Mirzaevich:
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