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American Journal of Drug Discovery and Development

Year: 2014 | Volume: 4 | Issue: 1 | Page No.: 80-84
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Authors

A. Carnot

J.S. Guerra

T.S. Souza

L.C. Carneiro

L.C. Carneiro

Keywords

Research Article

Antimicrobial Resistance and Plasmid Characterization of Escherichia coli Isolated in In natura Water

A. Carnot, J.S. Guerra, T.S. Souza and L.C. Carneiro L.C. Carneiro's LiveDNA
The constant use of antibiotics in human and animal medicines is causing a rise in Escherichia coli resistance to these medicines, interfering with the effective treatment of infections caused by this agent. For this study, we isolated and identified E. coli from in nature water samples and verified the presence of plasmids that caused bacteria resistance. We collated 24 water samples from two different stations (a water treatment station and a water captation station). From the contaminated samples, we evaluated antibiotic resistance to amoxicillin (5 μg) cephalexin (5 μg), azitromicin (5 μg), ampicilin (5 μg), tetracycline (5 μg) and ciprofloxacin (10 μg). We also evaluated whether the resistance was plasmidial or chromosomal. The results showed a frequent occurrence of multiple resistance to the main antimicrobials utilized, includingcephalexin (67.44%), amoxicilin (62.79%), ampicilin (58.13%), tetracycline (37.20%), azithromycin (32.55%), ciproflaxacin (18.60%). We observed the presence of different plasmidial profiles, including occurrences of amoxicillin (30%), ampicillin (30%), tetracycline (30%) and ciprofloxacin (10%). The study showed that the samples presented plasmids with genes resistant to important antibiotics used for public health.
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