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Asian Journal of Crop Science

Year: 2017 | Volume: 9 | Issue: 4 | Page No.: 141-148
DOI: 10.3923/ajcs.2017.141.148

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Authors


Dedi  Ruswandi

Dedi Ruswandi

LiveDNA: 62.18773

Budi Waluyo

Country: Indonesia

Andi Takdir Makkulawu

Country: Indonesia

Elia Azizah

Country: Indonesia

Yuyun Yuwariah

Country: Indonesia

Neni Rostini

Country: Indonesia

Keywords


  • albizia system
  • maize
  • Indonesian core collection
  • shade tolerant,
  • tropical maize
Research Article

Simple Sequence Repeats Analysis of New Indonesian Maize Inbred

Dedi Ruswandi Dedi  Ruswandi's LiveDNA, Budi Waluyo, Andi Takdir Makkulawu, Elia Azizah, Yuyun Yuwariah and Neni Rostini
Background and Objective: The assessment of the genetic diversity of new inbred lines has proven significant to the improvement of maize varieties. The objective of the study was to evaluate the diversity and relatedness of new Indonesian elite maize lines. Materials and Methods: Genetic diversity of 48 Indonesian elite maize lines was studied using six SSR markers. Some parameters were estimated including genetic relationship and genetic diversity. Genetic relationship was assessed using Jaccard’s similarity coefficient and was used to draw a neighbourhood-joining tree (NJT) by circle cluster using MEGA-pc software. Principal coordinate analysis (PCoA) was used for the estimation of genetic diversity. Results: All SSR loci detected in all genotypes were polymorphic. The population in this study is diverse as estimated by its polymorphic index coefficient (PIC). Population of 20 Indonesian maize lines was divided into 3 sub-groups, whereas 28 of mutant population was clustered into 7 sub-groups. Conclusion: SSR marker is a meaningful marker for genetic diversity study of Indonesian maize core collection. The forty-eight Indonesian maize genotypes were clustered into three major groups. These findings can be used in breeding methods for the development of populations.
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How to cite this article

Dedi Ruswandi, Budi Waluyo, Andi Takdir Makkulawu, Elia Azizah, Yuyun Yuwariah and Neni Rostini, 2017. Simple Sequence Repeats Analysis of New Indonesian Maize Inbred. Asian Journal of Crop Science, 9: 141-148.

DOI: 10.3923/ajcs.2017.141.148

URL: https://scialert.net/abstract/?doi=ajcs.2017.141.148

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