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Asian Journal of Biological Sciences
  Year: 2019 | Volume: 12 | Issue: 3 | Page No.: 499-505
DOI: 10.3923/ajbs.2019.499.505
 
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Characterization of Anti-oxidant and Anti-microbial Properties of Tamarindus indica Bark Extract on Various Pathogenic Micro-organisms
Utkarsh Tyagi , Surya Prakash Dwivedi and Sovan Bagchi

Abstract:
Background and Objectives: Prevention of food spoilage is usually achieved by use of chemical preservatives which have negative impacts human health. Due to such concerns it is necessary to find some natural preservatives. Within this text, the present study was designed to investigate the in vitro anti-microbial activities, MIC, MBC, phytochemical analysis and anti-oxidant activity of the acetone extract of bark of Tamarindus indica against the pathogenic micro-organisms. Materials and Methods: The in vitro anti-bacterial and anti-fungal activity was performed by agar well diffusion method while the phytochemical analysis was investigated by different methods like Mayer’s test, Ninhydrin test, Alkaline Reagent test, Ferric Chloride test, Froth test, Xanthoprotic test and Liebermann Burchard test. Antioxidant property was evaluated by DPPH method. Results: The T. indica bark shows the presence of phytochemicals like, phenolic compounds, tannins and steroids. The anti-microbial activity results were expressed as the average diameter of zone of inhibition. The acetone extract showed better anti-bacterial activity and with zone of inhibition (28-30 mm) and showed no anti-fungal activity when compared with other tested standard anti-biotic streptomycin (300 ppm) and trizol solution. The MIC of the acetone extracts ranged from 3.125-6.25 μg mL–1. However, MBC ranges from 6.25-12.5 μg mL–1 Extracts of T. indica bark also exhibited significant anti-oxidant activity, thus establishing the extracts as an anti-oxidant. Conclusion: The results obtained in this study give some scientific support to the T. indica bark for further investigation of compounds and in future could be used as drug and combinations of extracts may be the best herbal antibiotics.
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How to cite this article:

Utkarsh Tyagi, Surya Prakash Dwivedi and Sovan Bagchi, 2019. Characterization of Anti-oxidant and Anti-microbial Properties of Tamarindus indica Bark Extract on Various Pathogenic Micro-organisms. Asian Journal of Biological Sciences, 12: 499-505.

DOI: 10.3923/ajbs.2019.499.505

URL: https://scialert.net/abstract/?doi=ajbs.2019.499.505

 
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