Subscribe Now Subscribe Today
Science Alert
Curve Top
Asian Journal of Biological Sciences
  Year: 2013 | Volume: 6 | Issue: 3 | Page No.: 168-174
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Correlation of Traditional Knowledge with Laboratory Based Salmonella Detection in Egg Shell and Contents of Market Sold Egg

Anbessa Dabassa and Shiferaw Demissie

Salmonelosis is a leading food borne disease worldwide and Africa at large. Eggs were one of the sources Salmonella for the food. So, this study was aimed to evaluate the traditional knowledge with scientific methods for detection of Salmonella in egg shell and content. Randomly collected egg samples were categorized based upon traditional knowledge and Salmonella metabolic recovery and proliferation of cells were done with primary and secondary enrichment media. From a total of 60 samples analyzed, 31 isolates of Salmonella were detected. Moreover, Conventional biochemical and serological test methods were used to identify the suspected Salmonella. The isolates were checked for their susceptibility to different antibiotics on Muller-Hinton agar in standardized inoculum and disc diffusion technique with 11 Oxoid drug discs. The result of study shows that 71% and 29% of Salmonella isolates were detected from egg shell and contents, respectively. All the isolates were resistant to ampicillin; Amoxicillin, Tetracycline and also 45% of the isolates were susceptible to Gentamicin and 16% for ciprofloxacin. Most Salmonella isolates show multi-drug resistance for at least five antibiotics. The laboratory results were supported the traditional egg spoilage identification knowledge, hence most of Salmonella isolates were detected from movable, floating and opaque eggs. On the other hand, the detection of high number of Salmonella isolate from the egg shell could be due to poor sanitation of the laid egg environment. Hence, the appropriate care and awareness should be needed before and/or after the egg laid to minimize contamination of egg by Salmonella.
PDF Fulltext XML References Citation Report Citation
  •    Evaluation of Physico-chemical and Antimicrobial Susceptibility Patterns of Microorganisms Isolated from Awetu River, Jimma Town
  •    Identification, Characterization and Antibiotic Susceptibility of Salmonella and Shigella species Isolated from Blood and Stool Samples of Patients Visiting N. I. H, Islamabad
How to cite this article:

Anbessa Dabassa and Shiferaw Demissie, 2013. Correlation of Traditional Knowledge with Laboratory Based Salmonella Detection in Egg Shell and Contents of Market Sold Egg. Asian Journal of Biological Sciences, 6: 168-174.




Curve Bottom