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Asian Journal of Biological Sciences
  Year: 2009 | Volume: 2 | Issue: 4 | Page No.: 112-117
DOI: 10.3923/ajbs.2009.112.117
 
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Isolation of Stigmasterol and γ-Sitosterol from Petroleum Ether Extract of Woody Stem of Abelmoschus manihot

P.S. Jain, S.B. Bari and S.J. Surana

Abstract:
The aim of this study is to identify and characterize the bioactive principles from the woody stem of Abelmoschus manihot. It has wide folk medicinal use. For isolation of the compounds, the dried woody stem’s powder of Abelmoschus manihot was subjected to hot extraction with petroleum ether, this extract was saponified with alcoholic KOH and subjected to chromatography. Two compounds (PEA-2 and PEA-3) were isolated and purified by chloroform. Mass spectrum of PEA-2 and PEA-3 showed a parent molecular ion [M+] peak at mlz 412 which corresponds to the molecular formula C29H48O and 414 corresponds to C29H50O. In lH-NMR spectrum of PEA-2, H-3 proton appeared as a triplet of a double doublet (tdd) at S 3.62 and H-6 olefinic proton showed a multiplet at S 5.14. Two olefenic protons appeared downfield at S 4.16 (m) and S 4.14 (m) and in the 1H-NMR data of PEA-3, H-3 proton appeared at S 3.51 as a triplet of a double doublet with a J value of 4.5 and 1.1 MHZ and H-6 olefinic proton showed a multiplet at S 5.10. From the physical, chemical and spectral characteristics, PEA-2 and PEA-3 were concluded as stigmasterol and γ-sitosterol.
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How to cite this article:

P.S. Jain, S.B. Bari and S.J. Surana, 2009. Isolation of Stigmasterol and γ-Sitosterol from Petroleum Ether Extract of Woody Stem of Abelmoschus manihot. Asian Journal of Biological Sciences, 2: 112-117.

DOI: 10.3923/ajbs.2009.112.117

URL: https://scialert.net/abstract/?doi=ajbs.2009.112.117

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