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American Journal of Biochemistry and Molecular Biology
  Year: 2011 | Volume: 1 | Issue: 4 | Page No.: 349-358
DOI: 10.3923/ajbmb.2011.349.358
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Amino Acid Sequence of an Adipokinetic Neuropeptide from the Plant Bug, Iphita limbata Stal

A.P. Ajaykumar and M. Gokuldas

Intermediary metabolism in insects is regulated by small neuropeptides of the adipokinetic hormone/red pigment concentrating hormone family (AKH/RPCH). AKHs are synthesized and stored in the endogenous neurosecretory cells of Corpora Cardiaca (CC). The present investigation was carried out to elucidate the primary structure of adipokinetic neuropeptides in the plant bug, Iphita limbata using HPLC, bioassay and mass spectrometric analyses (MALDI-MS and MS/MS). Separation of an extract of retrocerebral complexes of I. limbata, by RP-HPLC and monitoring at 210 nm showed six distinctive absorptive peaks. The chromatographic fractions were examined for adipokinetic activity by a homologous in vivo bioassay. Two UV absorbance peaks were found to contain compounds that are significantly active in mobilization of lipids from the fat body. Comparison of HPLC profile of synthetic peptide, Pyrap-AKH with that of the extract, showed that the peptide had identical retention time as that of material in the extract with significant hyperlipaemic effect. MALDI-MS analysis of the corpora cardiaca extract indicated that the molecular mass 1000.4 Da of the ion peak obtained is similar to that of Pyrap-AKH. MALDI-MS/MS analysis in PSD mode confirmed the primary structure of adipokinetic hormone of I. limbata is pE-L-N-F-T-P-N-W-NH2 which is same as that of Pyrap-AKH. This data may be helpful for tracing the phylogeny of the insect order Heteroptera.
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How to cite this article:

A.P. Ajaykumar and M. Gokuldas, 2011. Amino Acid Sequence of an Adipokinetic Neuropeptide from the Plant Bug, Iphita limbata Stal. American Journal of Biochemistry and Molecular Biology, 1: 349-358.

DOI: 10.3923/ajbmb.2011.349.358






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