
	American Journal of Biochemistry and Molecular Biology
	Year: 2011 \| Volume: 1 \| Issue: 4 \| Page No.: 349-358 DOI: 10.3923/ajbmb.2011.349.358

Amino Acid Sequence of an Adipokinetic Neuropeptide from the Plant Bug, Iphita limbata Stal

A.P. Ajaykumar and M. Gokuldas

Abstract: Intermediary metabolism in insects is regulated by small neuropeptides of the adipokinetic hormone/red pigment concentrating hormone family (AKH/RPCH). AKHs are synthesized and stored in the endogenous neurosecretory cells of Corpora Cardiaca (CC). The present investigation was carried out to elucidate the primary structure of adipokinetic neuropeptides in the plant bug, Iphita limbata using HPLC, bioassay and mass spectrometric analyses (MALDI-MS and MS/MS). Separation of an extract of retrocerebral complexes of I. limbata, by RP-HPLC and monitoring at 210 nm showed six distinctive absorptive peaks. The chromatographic fractions were examined for adipokinetic activity by a homologous in vivo bioassay. Two UV absorbance peaks were found to contain compounds that are significantly active in mobilization of lipids from the fat body. Comparison of HPLC profile of synthetic peptide, Pyrap-AKH with that of the extract, showed that the peptide had identical retention time as that of material in the extract with significant hyperlipaemic effect. MALDI-MS analysis of the corpora cardiaca extract indicated that the molecular mass 1000.4 Da of the ion peak obtained is similar to that of Pyrap-AKH. MALDI-MS/MS analysis in PSD mode confirmed the primary structure of adipokinetic hormone of I. limbata is pE-L-N-F-T-P-N-W-NH₂ which is same as that of Pyrap-AKH. This data may be helpful for tracing the phylogeny of the insect order Heteroptera.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Identification and Structural Characterization of a Hyperlipemic Neuropeptide from the Mango Leaf Webber, Orthaga exvinacea Hampson

•	Elucidation of the Primary Structure of an Adipokinetic Neuropeptide from the Coffee Locust, Aularches miliaris L. (Pyrgomorphidae: Orthoptera)

•	Molecular Fingerprinting of Certain Cereal Aphids in Egypt (Hemiptera: Sternorrhyncha: Aphididae) Using RAPD and ISSRs Markers

•	Molecular Phylogeny of Horseshoe Crab

•	Evidence for an Autonomic Nervous System in Decapod Crustaceans

•	Activity of Hyperglycemic Hormone in the Eyestalk of Commercially Important Crab Portunus pelagicus (Linnaeus)

How to cite this article:

A.P. Ajaykumar and M. Gokuldas, 2011. Amino Acid Sequence of an Adipokinetic Neuropeptide from the Plant Bug, Iphita limbata Stal. American Journal of Biochemistry and Molecular Biology, 1: 349-358.

DOI: 10.3923/ajbmb.2011.349.358

URL: https://scialert.net/abstract/?doi=ajbmb.2011.349.358

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Chemical Abstract Services
		Google Scholar

Science Alert