Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Asian Journal of Biotechnology
  Year: 2011 | Volume: 3 | Issue: 4 | Page No.: 357-367
DOI: 10.3923/ajbkr.2011.357.367
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Regeneration Capacity of Cell Suspension Culture in Malaysian Rice Genotypes under Salinity Stress

N.N. Htwe, M. Maziah, H.C. Ling, F.Q. Zaman and A.M. Zain

Abstract:
Rice, a salt-sensitive species has a substantial genetic variation for salt tolerance within the cultivated gene pool. This study describes the establishment of cell suspension culture system and analysis of proline content of the selected rice genotypes under in vitro salt stress. The cell suspension cultures, initiated from the friable seed-derived callus, were established in MS medium supplemented with different concentrations of 2,4-D (0-25 μM) and subjected to different concentrations of NaCl (0-250 mM). The average Settled Cell Volume (SCV) of different clumps sizes was recorded. The results showed that MR219-4 exhibited the highest cell growth rate and regeneration capacity. The 750 μm sieved clumps have higher cell mass than the 500 and 250 μm sieved cell size. The shoot-regeneration capacity was maximum in 1/2 MS medium supplemented with 10 μM BAP. However, low regeneration capacity was obtained in NaCl treatments. MR219 (control) and MR219-9 appeared to be more NaCl tolerant than MR219-4. In addition, all genotypes significantly synthesized proline under NaCl conditions as demonstrated by the proline accumulation and MR219-4 revealed the lowest content among genotypes studied. These genotypes can be good model systems for studying the physiological mechanisms associated with in vitro selection for salt stress. This finding also suggests that proline may play a crucial role in protecting rice cells under salinity stress.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Induction of Salt Tolerance in Rice Through Mutation Breeding
How to cite this article:

N.N. Htwe, M. Maziah, H.C. Ling, F.Q. Zaman and A.M. Zain, 2011. Regeneration Capacity of Cell Suspension Culture in Malaysian Rice Genotypes under Salinity Stress. Asian Journal of Biotechnology, 3: 357-367.

DOI: 10.3923/ajbkr.2011.357.367

URL: https://scialert.net/abstract/?doi=ajbkr.2011.357.367

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom