Submit Manuscript

Asian Journal of Biotechnology

Year: 2011 | Volume: 3 | Issue: 2 | Page No.: 165-176
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 352

Search

Authors

Bidhan Roy

Asit B. Mandal

Keywords

Research Article

Profuse Microtillering of Androgenic Plantlets of Elite Indica Rice Variety IR 72

Bidhan Roy and Asit B. Mandal
The aim of this experiment was to induce profused microtillering of androgenic plantlets of elite indica rice (Oryza sativa L.) variety IR 72. Two microtillers were inoculated in each culture tube containing different medium and growth regulators. Various combinations of growth regulators were also used with each culture medium. Microtillering ability were noted on 45th day after inoculation of microtillers. MS medium with 4-8 mg L-1 of kinetin showed very fast microshooting with maximum of 94.7 shootlets per cultured shoot within 45 days of inoculation. Conversely, BAP at high dose (4-8 mg L-1) was found to exert inhibitory effects on shoot development, but induced large number of dormant embryos and pro-embryos. The caulogenic shoots that emerged from the base of the explants were successfully induced root on MS supplemented with 1 mg L-1 IAA. Evaluation of clonal fidelity of the microshoots through profiling of isozyme in respect of peroxidase and malate dehydrogenase and RAPD analysis was done to confirm true-to-type nature of the micropropagated plants. Microtillering and subsequent whole plant development may offer ample scope to develop large number of clones from a stock of choice for in vitro conservation, characterization and screening for diverse characters of agronomic importance. This approach may have far reaching consequences in transgenic development especially through direct bombardment of miniature shootlets en route biolistic and their subsequent multiplication en masse.
PDF Fulltext XML References Citation

Related Articles

Anther Culture Response in Boro Rice Hybrids
Study of Physiological Potentiality of Callus and Plant Regeneration through Somatic Embryogenesis in Rice Variety Swarna
Genetic Engineering for Abiotic Stress Tolerance in Agricultural Crops
Genetic Stability in Date Palm Micropropagation
Effect of Genotype and Callus Induction Medium on Green Plant Regeneration from Anther of Nepalese Rice Cultivars
In vitro Plant Regeneration Through Anther Culture of Some Iranian Local Rice (Oryza sativa L.) Cultivars

Leave a Comment

Your email address will not be published. Required fields are marked *