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Asian Journal of Biotechnology
  Year: 2010 | Volume: 2 | Issue: 1 | Page No.: 27-36
DOI: 10.3923/ajbkr.2010.27.36
 
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Extra Cellular Endoglucanase Production by Rhizopus oryzae in Solid and Liquid State Fermentation of Agro Wastes

M. Karmakar and R.R. Ray

Abstract:
Extra cellular endoglucanase was found to be produced by Rhizopus oryzae PR7 from both liquid and solid state fermentations of various agro wastes. Among the 18 types of wastes tested, orange peel, dried flower and sugar cane bagasse showed promising results. The highest amount of enzyme production was obtained at 48 h of growth at 37°C at static condition. The Liquid State Fermentation (LSF) was found to be better than Solid State Fermentation (SSF) of the wastes for extra cellular endoglucanase synthesis. The pH preferred for enzyme production was found to vary with the nature of cellulosic waste used. The waste substrate at a concentration of 0.75% (w/v) gave the best result, whereas ammonium sulfate was proved to be the most effective nitrogen source for enzyme production. The alkali and acid pretreatment of the agro wastes could not increase enzyme production, while double sterilization enhanced the enzyme production only to a certain extent. The ability to produce high amount of endoglucanase within a very short period of 48 h and the capability of degrading wastes made the strain suitable for commercial production of the enzyme.

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How to cite this article:

M. Karmakar and R.R. Ray, 2010. Extra Cellular Endoglucanase Production by Rhizopus oryzae in Solid and Liquid State Fermentation of Agro Wastes. Asian Journal of Biotechnology, 2: 27-36.

DOI: 10.3923/ajbkr.2010.27.36

URL: https://scialert.net/abstract/?doi=ajbkr.2010.27.36

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