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Asian Journal of Biotechnology

Year: 2010 | Volume: 2 | Issue: 3 | Page No.: 159-168
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Authors

A.H. Gorji

F. Darvish

M. Esmaeilzadehmoghadam

F. Azizi

Keywords

Research Article

Application RAPD Technique for Recognition Genotypes Tolerant to Drought in some of Bread Wheat

A.H. Gorji, F. Darvish, M. Esmaeilzadehmoghadam and F. Azizi
The objective of this study was to provide selection criteria for drought tolerance by RAPD technique. Common wheat (Triticum aestivum L.) is a grass species, cultivated world wide. Genetic diversity evaluation of germplasm is the basis of improvement in wheat. The complexity of the wheat genome has delayed the development and application of molecular markers to this species and wheat now lies behind barley, maize and rice in marker development. However, improvements in marker detection systems and in the techniques used to identify markers linked to useful traits has allowed considerable advances to be made in recent years. The use of PCR based assays having advantage of being quick, easy to use and refractory to many environmental influences can complement traditional methods of germplasm characterization. The RAPD markers were used to determine the genetic differences between the 30 bread wheat genotypes and to determine the molecular markers associated with tolerance to drought.The present study found that RAPD analysis is a valuable diagnostic tool. Different sets of RAPD primer were used to study the polymorphism at molecular level. Initial screens involved growing 20 genotypes at seedling stage under drought conditions (-5 and -8 bar exerted by PEG 6000 in ahydroponic experiment). These tests confirmed the tolerance of the 6 above mentioned genotypes. Thirty 10-mer RAPD primers were used for fingerprinting of the genotypes of which primers P16 (TCGGCGGTTC) and P17 (CTGCATCGTG) produced, respectively a 900 and a 750 bp band present in drought tolerant (absent in others) genotypes. The results substantiate the use of these DNA markers in germplasm screening for drought tolerance in wheat.
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Comments

Shatha A Yousif Reply

The same results as mention by Pakniyat and Tavakol (RAPD markers associated with drought tolerance in bread wheat (Triticum aestivum L.).Pak J Biol Sci. 2007 Sep 15;10(18):3237-9). they found that the RAPD primers P6 (TCGGCGGTTC) and P7 (CTGCATCGTG) produced respectively a 920 and a 750 bp band present in drought tolerant (absent in others) cultivars. p6 and p7 the same sequnces of the primers P16 (TCGGCGGTTC) and P17 (CTGCATCGTG) that you used in your paper

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