Abstract:
A protocol for callus induction and shoot multiplication was developed for leaf and nodal segments of Memecylon edule (Melastomataceae). Leaf and nodal explants were cultured on MS medium supplemented with different phytohormones combinations IAA, NAA, 2,4,D, BAP, KIN, GA3 with different concentrations (0.5-6 mg) for callus induction and shoot multiplication. Callus was initiated from cut ends of green tender leaves and nodal segments of Memecylon edule in both dark and light condition. Green friable callus and light brown calli was derived from nodal segments of MS ½ strength medium supplemented with 0.7% agar and 30% sucrose with GA3+BAP+NAA, BAP+NAA+KN+GA3, BAP+KN+NAA, BAP+GA3+NAA, BAP+GA3+2,4,D, IAA+NAA+ 2,4,D+KN+BAP, BAP+KN+NAA+2,4,D+GA3 promotes the callogenesis in nodal explants. A best combination for shoot multiplication was MS medium supplemented with IAA+BAP+GA3 from the nodal segments and the nodal calli and IAA+BAP combination with 1 to 3 mg concentration was best for leaf callus. Percentages of callus induction were higher in nodal segments than leaf. In both leaf and nodal explants callus was developed in the MS medium containing BAP with IAA and GA3 combinations.
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