
	Asian Journal of Biochemistry
	Year: 2014 \| Volume: 9 \| Issue: 2 \| Page No.: 86-97 DOI: 10.3923/ajb.2014.86.97

Hepatocyte Oxidative Stress Indicators of Carbon Tetrachloride Induced Hyperlipidemic Rats (Rattus norvegicus) Treated with Allium sativa Extract

Chikezie Paul Chidoka and Uwakwe Augustine Amadikwa

Abstract: Pathogenesis of several chronic liver diseases has been attributed to overwhelmed antioxidant protective system against Reactive Oxygen Species (ROS). The present study ascertained the capacity of short-term administration of ethanolic extract of Allium sativa to neutralize ROS and ameliorate hyperlipidemia. Hyperlipidemia was induced in rats by single intra-peritoneal injection of CCl₄ (dosage = 2.0 mL kg^-1), followed by treatment with ethanolic extract of A. sativa (dosage: 200 and 400 mg kg^-1) at a regular interval of 16 h for 64 h. Blood samples were drawn from the rats at t = 0 and t = 76 h, i.e., 12 h after the end of 64 h treatment with CCl₄ per A. sativa extract treatment, to ascertain hepatic function and Serum Lipid Profile (SLP). In addition, liver post mitochondrial supernatant (PMS) fraction was measured for oxidative stress indicators: Lipid peroxidation (LPOx), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and reduced glutathione (GSH). On the average, short-term administration of ethanolic extract of A. sativa caused reduction of SLP in the following magnitude: Total cholesterol (TC) = 19.48, triacylglycerol (TAG) = 48.59, VLDL-C = 48.57, LDL-C = 19.49 and increase in HDL-C = 32.43%. Also, improvement in oxidative stress indicators gave SOD = 10.20, GPx = 30.92, CAT = 18.18, LPOx = 35.92% and GSH = 51.09%. Although the administration of A. sativa extract to the rats did not restore full therapeutic benefits within the experimental time (t = 76 h), the capacity of the plant extract to ameliorate oxidative stress and hyperlipidemia in the animals was fairly at par with the standard hepatic drug-hepaticum.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Oxidative Stress Indicators of Human Sickle Erythrocytes Incubated in Aqueous Extracts of Three Medicinal Plants

How to cite this article:

Chikezie Paul Chidoka and Uwakwe Augustine Amadikwa, 2014. Hepatocyte Oxidative Stress Indicators of Carbon Tetrachloride Induced Hyperlipidemic Rats (Rattus norvegicus) Treated with Allium sativa Extract. Asian Journal of Biochemistry, 9: 86-97.

DOI: 10.3923/ajb.2014.86.97

URL: https://scialert.net/abstract/?doi=ajb.2014.86.97

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		FSTA
		Google Scholar

Science Alert