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Asian Journal of Biochemistry
  Year: 2011 | Volume: 6 | Issue: 3 | Page No.: 263-272
DOI: 10.3923/ajb.2011.263.272
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L-Arginine Ameliorates Arylesterase/Paraoxonase Activity of Paraoxonase-1 in Hypercholesterolemic Rats

Gamal El-din I. Harisa

The present study was conducted to investigate the effect of L-Arginine (Arg) supplementation on protein oxidation as well as Paraoxonase (PON) activities in rats received either normal or high cholesterol diet with or without 3% Arg in drinking water. Hypercholesterolemia (HC) was induced in rats by feeding diet containing cholesterol 5%, cholic acid 1% and propylthiouracil 0.5%. The spectrophotometric analysis methods were used for determination of lipid profiles as well as oxidative stress biomarkers in the plasma and liver tissues of rats. The results of current study revealed that the feeding of rats with Hypercholesterolemic Diet (HCD) resulted in significant increased of Atherogenic Index (AI) by 256% in respect to control rats. Arg supplementation to rats with HCD resulted in decrease of AI by 152% comparison with rats received HCD. Moreover, administration of Arg with normal or HCD attenuates the protein oxidation as well as lipids peroxidation in compared to intake of HCD alone. As a result of HCD feeding, plasma arylesterase (ARE) activity was decreased by 30% while in liver it decreased by 36%. Furthermore, paraoxonase activity (PON1) was decreased 42 and 74% for plasma and liver, respectively. On the other hand, the treatment with Arg ameliorates both activities in the plasma and liver in comparison with HCD. Arg preserve thiols and nitric oxide level, thereby reducing oxidative stress associated with increased cholesterol level. This may be related antioxidant effect of this amino acid. Therefore, Arg has beneficial effects in the treatment of HC thruogh decreases the proteins oxidation as well as preservation of high density lipoprotein function through maintaining PON activity.
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  •    Effect of L-Arginine on Some Biochemical Markers of Metabolic Syndrome Associated with Brain Function in Female Wistar Rats
  •    Effect of L-arginine on Some Anthropometric Parameters of Metabolic Syndrome in Normal Female Wistar Rats
  •    Influence of L-arginine on the Heart Histology and Function Markers of Metabolic Syndrome in Female Wistar Albino Rats
  •    Alterations in the Liver Histology and Markers of Metabolic Syndrome Associated with Inflammation and Liver Damage in L-arginine Exposed Female Wistar Albino Rats
How to cite this article:

Gamal El-din I. Harisa , 2011. L-Arginine Ameliorates Arylesterase/Paraoxonase Activity of Paraoxonase-1 in Hypercholesterolemic Rats. Asian Journal of Biochemistry, 6: 263-272.

DOI: 10.3923/ajb.2011.263.272






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