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Asian Journal of Biochemistry
  Year: 2009 | Volume: 4 | Issue: 3 | Page No.: 88-98
DOI: 10.3923/ajb.2009.88.98
 
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Extraction, Characterization and Nutritional Properties of Two Varieties of Defatted Foxtail Millet Flour (Setaria italica L.) grown in China

M.T. Kamara, Z.H. Ming and Z. Kexue

Abstract:
In this study, we examined the various protein fractions and protein concentrates of two selected varieties (white and yellow) of foxtail millet grown in China. Characterized by amino acid analysis, differential scanning calorimetry (DSC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The protein content for the white increased after defatting though not significantly different (p<0.05) from 11.50 to 11.59 and the yellow decreased from 11.41 to 11.39. Fat content shows a significant decreased (p<0.05) after defatting from 2.38 to 0.41(white) and 2.90 to 0.66 (yellow). Prolamin yellow and glutelin white were the major fractions (38.8 and 47.2%, respectively), followed by albumin yellow and white as 2.6 and 1.5%, respectively and globulin yellow and white as 2.5 and 1.4%, respectively and the difference was significant (p<0.05) among the various protein fractions. Results showed a significant amount of amino acids with essential amino acids above the recommended amount by FAO/WHO for humans. Albumin white possessed the highest DSC result (Tp = 79.583°C, ΔH = 5.115 J g-1), glutelin white the lowest (Tp = 66.682°C, ΔH = 0.313 J g-1). Fractions and concentrates had molecular sizes below 14.0 and above 97. 0 kDa. Protein fractions and concentrates are potential as functional food ingredient.
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How to cite this article:

M.T. Kamara, Z.H. Ming and Z. Kexue, 2009. Extraction, Characterization and Nutritional Properties of Two Varieties of Defatted Foxtail Millet Flour (Setaria italica L.) grown in China. Asian Journal of Biochemistry, 4: 88-98.

DOI: 10.3923/ajb.2009.88.98

URL: https://scialert.net/abstract/?doi=ajb.2009.88.98

COMMENTS
20 May, 2020
Niharika Sachdev:
This is a kind request for permission to use SDS PAGE analysis Figures from the article
"Extraction, Characterization and Nutritional Properties of Two Varieties of Defatted Foxtail Millet Flour (Setaria italica L.) grown in China
M.T. Kamara, Z.H. Ming and Z. Kexue" for publishing a review article. Kindly guide through the procedure.
Thank you.
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