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Comparison of Single Intradermal Test, Gamma Interferon Assay and Indirect
ELISA for the Diagnosis of Tuberculosis in a Dairy Farm |
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D. Neeraja,
B.M. Veeregowda,
M. Sobha Rani,
D. Rathnamma,
R. Bhaskaran,
G. Leena,
S.H. Somshekhar,
M. Saminathan,
K. Dhama and S. Chakraborty |
Abstract:
Bovine tuberculosis (bTB), caused by Mycobacterium bovis which belongs
to Mycobacterium tuberculosis complex (MTC), is a globally distributed
zoonotic disease in cattle. The present study was conducted in a dairy herd
with the history of prevalence of bovine tuberculosis. Two Cell Mediated Immunity
(CMI) based tests, Single Intradermal Test (SID), gamma interferon (IFN-γ)
assay and a serological test (enzyme linked immunosorbant assay, ELISA) were
employed for the diagnosis of tuberculosis in 45 animals. Of these, 8 (17.77%)
were positive by SID test, 10 (22.22%) by interferon gamma assay and none of
the animals were found positive by ELISA. Both the CMI tests performed were
found better than the antibody detection. Between the CMI tests, IFN-γ
assay showed better sensitivity than the SID test; combination of tests showed
better detection of the bTB infection in animals. ELISA results indicated that
animals were still in progressive stages of infection and the sensitivity of
the tests depends on the stage of infection in the study subjects. As a whole,
26.67% of the animals tested in the farm were found to be positive and/or reactors
to tuberculosis. It indicates that both the CMI tests were better than those
targeting antibody detection. ELISA did not detect even a single animal as positive.
These results indicate that no single test is able to detect all the infected
animals and also not having 100 % sensitivity and specificity. So combination
of tests always can be better employed for the diagnosis of bovine tuberculosis.
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How to cite this article:
D. Neeraja, B.M. Veeregowda, M. Sobha Rani, D. Rathnamma, R. Bhaskaran, G. Leena, S.H. Somshekhar, M. Saminathan, K. Dhama and S. Chakraborty, 2014. Comparison of Single Intradermal Test, Gamma Interferon Assay and Indirect
ELISA for the Diagnosis of Tuberculosis in a Dairy Farm. Asian Journal of Animal and Veterinary Advances, 9: 593-598.
DOI: 10.3923/ajava.2014.593.598
URL: https://scialert.net/abstract/?doi=ajava.2014.593.598
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