Submit Manuscript

Asian Journal of Animal and Veterinary Advances

Year: 2014 | Volume: 9 | Issue: 5 | Page No.: 281-291
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 355

Authors

P.K. Nanda

P. Swain

S.K. Nayak

T. Behera

K. Dhama

Keywords

Research Article

Comparative Study on Enzymatic and Explant Method in Establishing Primary Culture from Different Cultivable Cells of Indian Major Carp, Cirrhinus mrigala

P.K. Nanda, P. Swain, S.K. Nayak, T. Behera and K. Dhama
Enzymatic method using trypsin to establish primary culture from various cells from heart, liver, kidney, brain, testis, ovary, fin and embryo of Indian Major Carp, Cirrhinus mrigala was investigated and compared with that of explant culture method. The dissociation time to obtain cells from individual tissues and the viability of cells for primary culture varied at 0.125 and 0.25% trypsin concentration. Single cell culture of trypsinised cells from testis, ovary and embryo showed good attachment irrespective of trypsin concentration. Cells from liver and heart were found to be sensitive to trypsin only at higher concentration (0.25%); whereas, no attachment/or proliferation was observed from cells of kidney, brain and fin due to sensitivity to trypsin at both the concentrations. In contrast to this method to obtain primary culture, explant culture from most of the tissues showed better attachment, resulting in subsequent growth and proliferation of cells forming monolayer. Overall, the explant culture of most of the tissues of C. mrigala was found to be suitable and survived more passages as compared to single cell culture obtained through trypsinisation. For obtaining primary culture from single cells, further investigations are desired to identify tissue specific enzymes, standardization of dose, duration and temperature of enzymatic treatment.
PDF Fulltext XML References Citation

Leave a Comment

Your email address will not be published. Required fields are marked *

Useful Links

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

© Copyright Science Alert. All Rights Reserved