Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Asian Journal of Animal and Veterinary Advances
  Year: 2011 | Volume: 6 | Issue: 7 | Page No.: 715-722
DOI: 10.3923/ajava.2011.715.722
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

The Polymorphisms of β2-Adrenergic Receptor Gene on two Cattle Breeds in China

Bing Han, Hui-Ling Zhang, Li Zeng, Bin Yang, Reyanggu Abula, Xiao-Li Xu and Yong Chen

Abstract:
The beta-adrenergic receptors (β-AR) are a class of metabotropic G protein-coupled receptors that responsible for the muscle growth, fat deposition or lactation characteristics of domestic animals. The aim of this article was to scan nucleotide mutations in partial coding region of β2-AR gene on cattle. In this study, the polymorphisms of β2-AR gene on cattle were detected by methods of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and DNA sequencing in two cattle breeds in China, Xinjiang Brown cattle and Chinese Holstein cows. The results revealed that there were two genotypes and homozygote variant was not found in the two breeds. The genotypic frequencies at the Sma I locus were significantly different between the two breeds. The wild-type allele was ascendancy in the two breeds but its allele frequency was distinctly lower in the Chinese Holstein cows than that in the Xinjiang Brown cattle. DNA alignment results showed that three single nucleotide polymorphisms (SNPs), C11A, G53A and C129T were found in 5-coding region of β2-AR gene. The C11A mutation resulted in disappearance of Sma I cleavage site. In addition, the C11A and G53A mutations caused two amino acid residues replacement at the 4th and 18th of β2-AR. However, the C129T mutation was a synonymous one. In conclusion, the heterozygous frequency in Chinese Holstein cows was obviously higher than that in the Xinjiang Brown cattle. The Sma I locus would be a potential genetic marker for lactation performance of cattle.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Advances in the Research of AMPK and its Subunit Genes
  •    Polymorphism of Prolactin Receptor Gene and its Association with Egg Production Traits in Erlang Mountainous Chicken
  •    The Polymorphism in 5 'Regulatory Region and Exon 13 of PRKAG3 Gene and its Distribution Pattern in Different Goat Breeds
  •    The Polymorphism of a Novel Mutation of KAP13.1 Gene and its Associations with Cashmere Traits on Xinjiang Local Goat Breed in China
  •    A Novel 15 bp Deletion Mutation at KAP16.5 Locus in Cashmere Goat of China
  •    A Novel Mutation at Exon 4 of IGF-1 Gene in Three Indigenous Goat Breeds in China
  •    Association of SNP in the ExonII of Leptin Gene with Milk and Reproduction Traits in Holstein Iranian Cows
How to cite this article:

Bing Han, Hui-Ling Zhang, Li Zeng, Bin Yang, Reyanggu Abula, Xiao-Li Xu and Yong Chen, 2011. The Polymorphisms of β2-Adrenergic Receptor Gene on two Cattle Breeds in China. Asian Journal of Animal and Veterinary Advances, 6: 715-722.

DOI: 10.3923/ajava.2011.715.722

URL: https://scialert.net/abstract/?doi=ajava.2011.715.722

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom