Statistical Assessment of Medium Components by Factorial Design and Surface Methodology of L-Asparaginase Production by Isolated Streptomyces radiopugnans MS1 in Submerged Fermentation using Tapioca Effluent

Research Article

Statistical Assessment of Medium Components by Factorial Design and Surface Methodology of L-Asparaginase Production by Isolated Streptomyces radiopugnans MS1 in Submerged Fermentation using Tapioca Effluent

M. Senthil Kumar, K. Selvam and Singaravel

Streptomyces radiopugnans MS1 was isolated from marine environment and identified as a potent strain for producing L-Asparaginase from Tapioca effluent an inexpensive substrate. To optimize the L-Asparaginase production of this bacterial strain, 23 fermentation variables were screened using a Plackett-Burman design and were then further optimized via response surface methodology based on a Box-/Behnken design. Four significant variables, i.e., Tapioca Effluent, Corn steep liquor, L-Aspaparagine and Areation, were selected. The optimum values of the tested variables by response surface methodology were; Tapioca Effluent, 5 (% v/v); Corn steep liquor, 2 (% w/v); L-Aspaparagine, 0.003 (% w/v); Aeration, 0.7 (vvm) found to be optimum for L-Asparaginase production. The experimental result (19.5 IU mL^-1) in a medium optimized for L-Asparaginase production was in good agreement with the predicted value of a quadratic model (19.8 IU mL^-1). A verification experiment was carried out to examine model validation and revealed more than 99% validity. The model was very satisfactory as the coefficient of determination was 0.99. L-Asparaginase production in the optimized medium (19.5±0.33 IU mL^-1) increased 4.81-fold over that of the basal medium (4.05±0.84 IU mL^-1) in the fermenter.

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M. Senthil Kumar, K. Selvam and Singaravel, 2012. Statistical Assessment of Medium Components by Factorial Design and Surface Methodology of L-Asparaginase Production by Isolated Streptomyces radiopugnans MS1 in Submerged Fermentation using Tapioca Effluent. Asian Journal of Applied Sciences, 5: 252-265.

DOI: 10.3923/ajaps.2012.252.265

URL: https://scialert.net/abstract/?doi=ajaps.2012.252.265

Asian Journal of Applied Sciences

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M. Senthil Kumar

K. Selvam

Singaravel

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Research Article

Statistical Assessment of Medium Components by Factorial Design and Surface Methodology of L-Asparaginase Production by Isolated Streptomyces radiopugnans MS1 in Submerged Fermentation using Tapioca Effluent

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