Abstract: Background and Objective: Elasmobranchs (Sharks, skates, rays) are frequently available marine fishes which serve as an ideal host for the cestode parasites. Paraorygmatobothrium floraformis (Southwell, 1912) Ruhnke, 2011 is a cestode comprising a ubiquitous and specious genus of elasmobranch parasites. This parasite was isolated from the spiral intestine of commercially available elasmobranch fish, Rhizopriondon acutus of the Nellore coast, Bay of Bengal. The main objective of this work is to evaluate the morphological criteria, molecular characterization of Paraorygmatobothrium floraformis using the 28S ribosomal DNA and to determine the phylogenetic position of this species with bioinformatics tools like BLAST and Mega 6. Materials and Methods: The present study is restricted to Nellore Coast, which has a coastline of 169 km. The milk shark, Rhizopriondon acutus was collected from the local fisherman and the nearby Coastal areas of Nellore to examine the cestodes from their spiral intestines. They were preserved in FAA (Formalin-10 mL, Alcohol-85 mL and Acetic acid-5 mL) for further morphological analysis. Also, few parasites were fixed in 95% molecular grade ethanol and placed at low temperature of -20̊C for further molecular analysis. Results: The morphological data was taken from the whole mounts. However, the obtained gene sequences of the cestode parasite were submitted to the Genbank. The gene sequence of the parasite was compared with the available gene sequences of other cestode parasite from other elasmobranchs in the Genbank. The pairwise alignment of gene sequences showed 80% similarity with the order Tetraphyllidea and 5-10% mismatches with the sequences of Phyllobothriidea family. Also, the host, Rhizoprionodon acutus and Nellore coast serve as new host record and locality records for Paraorygmatobothrium floraformis (Southwell, 1912) Ruhnke, 2011. Conclusion: Species identification is one of the major difficulties in marine fish parasites. Ribosomal DNA has been widely used for taxonomic studies. The study presents a small molecular phylogenetic analysis of the elasmobranch tapeworms of family phyllobothridea and order Tetraphyllidea. These types of analyses can reveal the relationship between different orders, families and species of the cestode parasites from the marine environment.