Abstract: The phylogenetic relationships and divergence among different subgroups of plant and animal D-type cyclin sequences are studied. The orthologous plant and animal D-type cyclin translated sequences are extracted from NCBI GenBank database categorically identified as D, D1, D2, D3 and D5 types. Multiple sequence alignment was carried out using Clustal W tool of EBI and phylogram was constructed. Further, distinct nodes of phylogram were identified and their pattern was studied using cyclin signature from Prosite database. The results were discussed in terms of the relationships among different subgroups of D-type cyclins forming distinct nodes of phylogram and the conservation of their patterns at each node.