Abstract: The present study aimed at determining Xanthomonas oryzae pv. oryzae pathogenic diversity. Therefore, the disease survey was conducted in three ecozones of Togo-Forest savanna transition, Forest and dry savanna zones and the diseased samples were collected, the bacteria isolated and characterized by testing their virulence on 21 rice genotypes. The results revealed the occurrence of the bacterial leaf blight in the three ecozones. High bacterial leaf blight incidence of 50-65% was observed in Forest savanna transition and Forest zones, while it was up to 70% in the dry savanna zone. The highest incidence (70%) was recorded in dry savanna zone and the lowest (25%) was frequently observed in Forest zone. Pathotyping analysis of 13 bacterial isolates from samples collected from the infected fields against 21 rice genotypes to isolate and characterize bacteria virulence was carried out. Thus, AMMI cluster analysis revealed the existence of 3 Pathotypes (Pt) among these isolates: PtA highly virulent has 1 isolate, PtB virulent was made up of 3 isolates and PtC moderately virulent was made up of 9 isolates. At ecozone the analysis revealed the presence of PtB and PtC in the Forest savanna transition zone, PtA and PtC in Forest zone and PtB and PtC in the dry savanna zone. The present results provided knowledge on the bacteria virulence and its structure across ecozones of Togo and provided useful information for selection of genotypes for durable resistance to the disease.