Abstract: Short postharvest vase life is one of the most important problems on the cut flowers. The aim of this study was to evaluate the efficacy of silicon, nickel and acetylsalicylic acid and their interaction on extending the vase life, total chlorophyll content, ACC-oxidase (Aminocyclopropanecarboxylate oxidase, ACO) activity, anthocyanin leakage, membrane stability and malondialdehyde (MDA) content of Cut rose flowers. The treatments were distilled water, silicon (0, 1.5, 2.5 mM), acetylsalicylic acid (0, 1,1.5 mM) and nickel (0, 1, 2 mM). Vase life in solution containing 1.5 mM silicon and 1 mM acetylsalicylic acid didn’t have significant difference than control. The results showed that silicon, acetylsalicylic acid and nickel treatments increased cut flower water absorption, fresh weight and vase life, while decreasing malondialdehyde content, ACC-oxidase activity and membrane premeability together with total delay of senescence and peroxidation of lipids. Our results suggest the application of silicon, acetylsalicylic acid and nickel in preservative solutions for rose flowers maintained the vase life of flowers for a longer period.