Abstract: The conventional approach make the insect based natural fibre, silk to create only textiles but, an insight on production pace, eco-friendliness and bio-compatibility have widen its potential for nutritional, cosmetic, pharmaceutical, bio-medical and bio-engineering functions. The promotion of silkworm as bio-factory to synthesize important bio-material (silk protein) useful for innovative and advanced functional biological applications is a big trend in modern applied research. The worth as protein-rich diet for cardiac and diabetic patients and potential ingredient for cosmetic preparations designates the nutritional and aesthetic value of silk protein. The anti-inflammatory, anti-tumefacient, anti-coagulant and bio-stability competence made silk protein a pharmaceutical, while the property of bio-resorbability serve the function of drug delivery. The bio-attuned capacity made silk fibre as an apt bio-engineering base for tissue wall and membrane repairs; muscle ligament, blood vessel and nerve gadget restoration; and tooth, cartilage and bone reconstruction. The sericin and fibroin proteins of silk are promising wound healing agents, anti-oxidant and bio-adhesive mediators, scaffolds and implants for tissue-supporting prosthetics of human body. The vital need of contemporary sericulture industry is to explore, realize and attain the comprehensive utility avenues of the silk protein to provide biological substitutes, biochemical and physical regulatory aspects for lost or damaged human functions, which simultaneously up-keep the prosperity of silk stake-holders.