Abstract: The isolated heparin and heparin-like Glycosaminoglycans (GAGs) from the cuttlefish Euprymna berryi was studied for the antimicrobial activity. The bacterial strains such as (Bacillus subtilis, Pseudomonas aeruginosa, Escheriachia coli, Staphylococcus aureus, Shigella flexineri) and fungal strains such as Aspergillus fumigatus Fusarium sp., Cryptococcus neoformans, Microsporium sp. and Candida albicans present study. various concentration (25, 50, 75 and 100%) used in this study. The heparin and heparin-like (GAGs) crude and purified sample showed activity against all the pathogenic bacterial strains. Whereas the antifungal activity in crude and purified sample was no activity against Microsporium sp. Fusarium sp. (crude) and Microsporium sp. (purified) respectively in all the concentration. The other all the fungal strains having activity in all the concentration. The activity of heparin and heparin-like GAGs extract was found to be higher in 100% concentration than the other concentration. In general the increasing concentration showed increasing activity of the extract. The heparin and heparin-like extract showing good antimicrobial activity are under going further analysis to identify the active constituents.