Abstract: Concentration of chromium and cadmium were investigated in the gill, gonads, skin and muscle tissues of six commercially edible fishes sampled from upper course of gangetic West Bengal. Heavy metal concentrations in the tissues tended to vary significantly among seasons and monsoon period showed particularly high metal concentration compared to pre-monsoon and post-monsoon period. Muscle tissues and gill showed higher concentration of chromium and cadmium than the gonads and skin. Highest concentration of chromium and cadmium was detected in gill tissues. Lowest concentration observed in gonads of fish sampled from upper course of the River Ganga. Analysis of the above two metals were also carried out in ambient aquatic and sediment phases to monitor the degree of pollution. Higher values of chromium and cadmium were observed in sediment phases in comparison to water and fish. Further metals were observed to become bio-accumulated. High degree of species-specificity was also observed for metal accumulation and in case of chromium accumulation rate was higher than that of cadmium. During study, influence of physico-chemical parameters like pH, Alkalinity and temperature were also investigated to find out if they have any influence on accumulation phenomenon of chromium and cadmium in the muscle tissue of the selected fish species.