Abstract: Landscape fragmentation has been identified as fundamental reason for biodiversity loss. Satellite remote sensing data and Geographic Information System have been used to spatially model the disturbance regimes and to integrate the ground based non-spatial data with the spatial characters of the landscape in Warangal district of Andhra Pradesh, India. Disturbance index has been computed for the study area by linearly combining fragmentation, porosity, interspersion, juxtaposition and proximity of road and settlements. Of the eight natural forest types, moist deciduous forests have shown low fragmentation (78.40% of area). Over all disturbance gradient analysis indicates 52.74% of the total forested areas are under low disturbance, followed by 28.04% under medium and 19.22% under high. The present approach of disturbance gradient analysis provides insight into the disturbance status of forest which is useful for forest management.