Abstract: Background and Objective: Potassium dichromate (K2Cr2O7) has been demonstrated to induce oxidative stress and carcinogenic in nature. This study was aimed to evaluate the protective effects of different solvent (aqueous, methanol and petroleum ether) extract of Andrographis paniculata on lipid peroxidation and antioxidants status against chromium-treated liver and lungs mitochondria. Materials and Methods: A group of male Wistar rats (80-100 g) were obtained and divided into eight groups. The animals of seven groups were induced K2Cr2O7 at a dose of 0.8 mg per 100 g body weight per day (20% LD50) for 28 days. The animals of six of the chromium treated groups injected different solvent extracts at a dose of 250 and 500 mg kg–1 b.wt., daily for a period of 28 days. The animals of the remaining group received only the vehicle (0.9% physiological saline), served as control. After completion of chromium-treatment the animals were sacrifice and intact liver and lungs were dissected out for further use. Results: Measurement of lipid peroxidation (MDA), conjugated dienes and antioxidants were used to monitor the antiperoxidative effects of different solvent extract in liver and lungs mitochondria. The increased lipid peroxides and conjugated dienes in liver and lungs of chromium-treated rats was accompanied by a significant decrease in the levels of glutathione (GSH and GSSG) and the activities of glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione-S-transferase (G-S-T), superoxide dismutase (SOD) and catalase (CAT). Conclusion: The results of the present study suggest that the administration of different solvent extract significantly supplement the lipid peroxidation and enhanced the antioxidant status.