Abstract: Background and Objective: Helicobaeter pylori is a common bacterial infections worldwide, and linked to induction of chronic atrophic gastritis in infected individuals, and subsequently proceeded to gastric carcinoma. The aim of study to determine the frequency rate of biomarkers positivity, family history of gastritis and drug usage between H. pylori positive and negative gastritis. Materials and Methods: Serum collected from 136 subjects of the 72 were H. pylori positive and 64 were H. pylori negative as control group. Pepsinogen A, pepsinogen C, CD44, gastrin and human interferon gamma in serum were detected using ELISA test. Results: Pepsinogen A frequency of positivity rate was higher in H. pylori negative cases (37.5%) than in those H. pylori positive cases (23.6%), but the difference was not significant. The frequency rate of pepsinogen C positivity was 68.6% in H. pylori positive group and 31.4% in H. pylori negative cases with significant difference. The frequency positivity rate of gastrin was 70.8% in H. pylori positive gastritis, while it was 50% in H. pylori negative cases and the difference was significant. The frequency positivity rate of CD44 was 80.6% in H. pylori positive gastritis, while it was 50% in H. pylori negative cases and the difference was significant. The frequency positivity rate of interferon gamma was 81.9% in H. pylori positive gastritis, while it was 50% H. pylori negative cases and the difference was significant. Conclusion: High frequency rate of low positive pepsinogen A, high positive rate of gastrin, CD44 and interferon gamma were predictive biomarkers for chronic gastritis.