Abstract: The effect of phenobarbital pretreatment on the toxicity of Calotropis procera latex to Nubian goats was examined. The goats receiving phenobarbital sodium at daily oral doses of 20 mg/kg/day for 14 days plus an oral dose of C. procera latex on day 15, exhibited marked depression, lateral deviation of the head and neck, dyspnea and frequent urination and histo-pathologically, they showed centrilobular hepatocellular necrosis and fatty cytoplasmic vacuolation of the hepatocytes. The activities of GGT, GDH, LDH, AST and ALP and the concentration of creatinine were higher in this group than the group receiving latex alone. The study showed that phenobarbital pretreatment was found to potentiate the effect of C. procera latex in Nubian goat’s kids.