Abstract: The present research was done for preliminary identification and serological detection of Brucella melitensis and Brucella abortus in Bengal Goats. For this purpose, samples were collected from stomach content of aborted fetus, uterine fluid, vaginal swab, blood serum and milk of 20 Bengal Goats from Bangladesh Livestock Research Institute (BLRI) goat farm, Dhaka and Lalmonirat goat farm that caused abortion. Samples were cultured and subcultured in Nutrient agar media and Blood agar media and incubated in aerobic and anaerobic incubator to obtain pure cultures of individual bacterium. As Brucella melitensis and Brucella abortus are strictly aerobic, colonies grown in aerobic incubator were subcultured to get the specific bacterium. Twelve pure isolates were obtained from aerobic growth and catalase tests were performed with those isolates and eight of them showed positive results. Then serum was collected from respective goats and serological test (serum agglutination test) was performed by using Brucella antigen (kit). Test results were negative showing the absence of Brucella melitensis and Brucella abortus into the serum samples. For further confirmation, Milk Ring test, Rose Bengal test, Complement Fixation test, ELISA test were performed with the samples of suspected goats and Brucella antigen (kit). All results from the tests imply the negativity of the presence of Brucella melitensis and Brucella abortus.