Abstract: Background and Objective: Malaysia needs approximately 200 t of seeds usually for the replanting programme in the rubber plantation. However, its behavior as recalcitrant seed has no dormancy characterization, short life span and the germination rate decreased over time have given a critical challenge to set up suitable storage condition. Improper storage condition affected the rootstock preparation for planting materials in rubber nursery and maintaining the biochemical nature of the seed in storage period is important to maintain seed longevity. Therefore, the study aim to determine the effect of storage temperature on the biochemical properties of Hevea seed and to evaluate the ability of the potential temperature condition to maintain the seed longevity. Materials and Methods: Fresh Hevea seeds were collected from RRIM Mini Station of Tok Dor. Three temperature conditions, i.e. 8, 13 and 27°C were applied as storage conditions. The data obtained were subjected to one-way analysis of variance (ANOVA) using statistics software Prism 5 and Excel. The level of significance of the differences between mean values was estimated by post-test of Newman-Keuls test. Results: The results showed that the temperature of 27°C was significantly affected the high loss of enzyme activity, high moisture decrease, high lipid and protein contents, high contamination in the seed, high nutrient loss and high ratio saturated to unsaturated fatty acid. Thus, it reflected the low germination rate and seed viability only maintained until 2 weeks of storage. Temperatures of 8 and 13°C can maintain the seed viability until 8 weeks storage but stored at 13°C produced the best results based on the higher germination, lower loss of enzyme activity, lower moisture decrease, low lipid and protein contents, no contamination in the seed, lower nutrient loss and lower ratio saturated to unsaturated fatty acid. Conclusion: The temperature of 13°C was the most suitable for storing the Hevea seeds.