Abstract: Quercus semecarpifolia is a multipurpose tree species but facing the problem of poor regeneration in natural habitat. The seeds of Q. semecarpifolia shows the recalcitrant behavior as the seeds shed at high moisture content, short viable, desiccation and chilling sensitive and skip the maturation drying stage. To understand the regeneration potential of the species, seeds were examined under different temperature and light/dark conditions in intact form and after scarification and stratification. The finding indicates that the light, temperature, scarification and stratification have considerable effect on regeneration of Q. semecarpifolia when tested for radicle emergence and seedling recruitment under laboratory conditions. Higher (35°C) as well as lower (5°C) temperature did not favour radicle emergence, its growth and seedling establishment; however 15°C is best suited temperature to emerge radicle and root shoot development at which around 91% seeds resulted into seedlings. Scarification of seeds hardly matters for enhancement of radicle growth and seedling production significantly but light matters a lot. Light exhibits highest (90%) and fastest radicle growth (within 10 days) as well as shoot emergence (within 30 days) and survival percentage if compared with the germination in dark. Prechilling treatment at 0°C lost total viability even within 24 h.