Abstract: Aromatic and medicinal plants are widespread throughout world. The experiment was started in season 2009-2010. In the present work, effect of exogenous application of glutamine (2 and 4 mM) and malic acid (150 and 200 mg L-1) on components of essential oils of Mentha spicata was evaluated. All glutamine and malic acid treatments enhanced α-pinene, sabinene, β-pinene, limonene, 1,8-cineole, menthone, menthol and terpin-4-ol, while tricyclene, α-thujene, β-myrcene, p-cymene, cis-ocimene, γ-terpinene and α-terpinolene decreased. The study demonstrated that glutamine and malic acid can be change secondary metabolites in Mentha spicata.