Abstract: The diversity of Aspergillus from soil of teak dominated kusmi forest an Indian subtropical teak forest of Gorakhpur, has been studied and compared with earlier reports. The nine species of Aspergillus namely A. aculeatus, A. carbonarius, A. deflectus, A. fischeri, A. niger, A. phoenicis, A. puniceus, A. sparsus and A. wentii were unaffected and uniformly distributed over the time period. There has been substantial loss of 12 species of Aspergillus namely A. carneus, A. deflectus, A. melleus, A. panamensis, A. petrakii, A. puniceus, A. quardilineatus, A. rugulosus, A. sparsus, A. subolivaceus, A. sulphureus and A. terricola which were reported in earlier study. A total of four species namely A. flavor-furcatis, A. oryzae, A. tubingensis and A. zonatus were supposed to be new because none of literature have reported earlier about those four. The shift in the diversity of Aspergillus might be due to changes in the vegetation of the forest over the time.