Abstract: Background and Objective: The prevalence of obesity is increasing both worldwide and locally in India. It is considered a major health problem and often leads to other associated diseases such as type 2 diabetes, ischemic heart diseases, stroke and cancer. In the present study, the effects of powder of Rosa damascena flowers on high-fat diet-induced obesity in Wistar albino rats were examined. Materials and Methods: Female Wistar albino rats fed with a High Fat Diet (HFD) (p.o) for 6 weeks were used to induce obesity. Powder of R. damascena (214 mg kg–1 b.wt.) petals administered orally to HFD-fed rats for 6 weeks. Physiological parameters like body weight, food and water intake, fat pad analysis and biochemical parameters like serum lipids, glucose, Serum Glutamate Oxaloacetate Transaminase (SGOT) and Serum Glutamate Pyruvate Transaminase (SGPT), serum urea and creatinine levels were measured. Results: Treatment with powder of R. damascena flower petals to HFD-induced obese rats resulted in a significant reduction in body weight gain, fat pads, serum lipids, glucose, SGOT, SGPT and creatinine levels as compared to rats fed HFD alone. Further, the extract also showed a significant increase in High-Density Lipoprotein (HDL) levels. Conclusion: These results exhibit that the R. damascena flower possesses significant anti-obesity potential.