Abstract: Antioxidant phytochemicals such as β-carotene and xanthophyll contents were estimated in three different medicinal plants, widely distributed in Kumaon Himalayas using a reverse-phase HPLC system. Maximum β-carotene content (481.57 mg/100 g) was found in the leaves of Asplenium dalhousiae and maximum xanthophyll content (678.61 mg/100 g) was found in the leaves of Osmanthus fragrans. The aim of this study was to characterize the antioxidant value of the medicinal plants with particular attention to carotenoids and xanthophyll.