Abstract: The antioxidant phytochemicals viz., α-carotene, β-carotene, xanthophyll and lycopene were estimated in fresh samples of tomato, bitter gourd, brinjal, bottle gourd, pumpkin, cucumber, pointed gourd, chilli, okra and radish at the edible maturity stage using reverse phase HPLC system. The mean value of α-carotene, β-carotene and xanthophyll were 0.611, 4.637 and 0.995 mg/100 g, respectively. The mean lycopene content in tomato was found 7.231 mg/100 g. The range of α-carotene, β-carotene and xanthophyll were 0.049-1.919, 0.721-12.34 and 0.047-2.545 mg/100 g, respectively. Results indicate that vegetables are relatively abundant source of natural antioxidants.