Abstract: In this study, the phytochemical analysis of Acanthus ilicifolius and Avicennia officinalis were studied. These created an interest to test the possible phytochemical activity of the leaves of these plants, which has not been reported. Acanthus ilicifolius and Avicennia officinalis leaves indicate the presence of Protein, Resin, Steroids, Tannins, Glycosides, Reducing sugar, Carbohydrates Saponnins, Sterols, Terpenoids, Phenol, Cardioglycosides and Catachol. However acidic compounds are absent in this plant. In the GC-MS analysis, 7 bioactive phytochemical compounds were identified in the methanol extract of Acanthus ilicifolius leaves and 3 bioactive phytochemical compounds were in the methanol extract of Avicennia officinalis. The Acanthus ilicifolius and Avicennia officinalis revealed the presence of medicinal active constituents by GC-MS. The functional group present in these compounds was identified by IR spectral analysis. This study also helped to identify the formula and structure of biomolecules which can be used as drugs.