Abstract: This study described phytochemical examination of flowers of Caesalpinia crista Linn. Four flavonoid compounds have been characterized as 3, 5, 7, 3', 4'-pentahydroxy flavanone-3-O-β-D-xylopyranosyl-7-O-α-L-arabinopyranosyl-(1→3)-O-α-L-rhamnopyranoside (A), 4'-hydroxy-5, 7-dimethoxy flavone-4'-O-β-D-xylopyranosyl-(1→3)-O-β-D-glucopyranosyl-(1→4)-O-α-L-rhamnopy ranoside (B) 5, 2'-dihydroxy-6, 7-dimethoxy isoflavone (C) and 3, 5, 7, 3', 4' ,5'-hexahydroxy flavone (D) by various colour reactions, chemical degradations and spectral analysis from this plant. Acid hydrolysis, permethylation and enzymatic hydrolysis of compounds were carried out for identification of compounds. In this study were found to be isolated for the first time in this plant species. Isolated compounds were screened against S. enteritidis, K. pneumoniae, P. aeruginosa and M. lutens, bacteria and antifungal activity against F. oxisporum, A. niger and P. digitatum fungi showed good activity. Streptomycin used as standard antibacterial agent and Griseofulvin used as standard antifungal agent.