Abstract: This study evaluated phytochemical and some biological activities of the aqueous methanolic extract of the leaves of Diospyros lotus L. Eight phenolic compounds were isolated and identified as gallic acid, methylgallate, ellagic acid, kaempferol, quercetin, myricetin, myricetin 3-O-β-glucuronide and myricetin 3-O-α-rhamnoside. The structures were established on the basis of chemical and spectral analysis. The extract was tested for antioxidant, analgesic, anti-inflammatory and hepatoprotective activities. The 70% methanolic extract of D. lotus up to 5 g kg-1 b. wt. revealed no obvious toxic effects in mice and its antioxidant activity showed a marked significant scavenging activity. On the other hand, the extract indicates a central and peripheral analgesic effect. The acute paw oedema response was significantly reduced. The protective effect of the extract against acetaminophen induced liver damage was indicated by the reduction of the elevation of the liver enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma glutamyItransferase (GGT) that caused by acetaminophen. The phenolic compounds isolated from Diospyros lotus in this study were found to be isolated for the first time from the genus Diospyros.