Abstract: Methanolic extract of Commiphora kerstingii Engl. stem bark was screened for antioxidant activity using DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging assay to obtain an IC50 value of 26.27±0.24 μg cm-3 compared to ascorbic acid with an IC50 value of 33.59±0.21 μg cm-3 used as control. Antibacterial activity against Bacillus subtilis and Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa and Pseudomonas fluorescens were tested. The result showed that Staphylococcus aureus was the most inhibited by the methanolic extract with diameter of inhibition zone of 30 mm while Pseudomonas fluorescens was the least inhibited with diameter of inhibition zone of 16 mm. Phytochemical investigation showed the presence of alkaloids, saponins, anthraquiniones, cardiac glycosides, tannins and flavonoids in the methanolic extract of Commiphora kerstingii stem bark.