Abstract: Chemical constituents investigation of Gazania splendens Moore (Compositae family) let to isolate two flavonoids via luteolin and luteolin 7-O-glucoside, 11 n-alkanes (C14-C30), β-amyrin, cholesterol, β-sitosterol, stigmasterol and eight fatty acids. The structures of these compounds were established by Mass Spectrometry (MS), Gas-Liquid Chromatography (GLC) and spectroscopic techniques, including Ultra-violet (UV), Infra-Red (IR). The percentage of α-cellulose content in the leaves and flowers of the plant were 70.50 and 73.11%, respectively, hence the percentage in whole plant was 71.81%.