Abstract: Background and Objective: Increased Malondialdehyde (MDA) will cause endothelial damage and dysfunction. Soluble fms-like tyrosine kinase-1 (sFlt1) is known increase in amniotic fluid in preeclamptic patients. This study was aimed to determine the relationship between MDA and sFlt1 with maternal and neonatal outcome in preeclampsia. Materials and Methods: This was an observational analytic study with cross sectional study design. The sample size was 35 samples of pregnant women with preeclampsia. Sampling was done by consecutive sampling, from obstetrician’s practice at RSUP. Dr. M. Djamil Padang, RSI. Ibnu Sina Padang and RSU. BMC Padang. After maternal and neonatal examination and measurements MDA and sFlt1 levels of cubital venous blood by using Enzyme-Linked Immunosorbent Assay (ELISA) method, Pearson correlation was performed to assess the relationship between variables for normally distributed data and Spearman’s correlation for abnormal distribution data with significance level p<0.05. Result: This study found MDA associations with maternal and neonatal outcomes were systolic blood pressure, diastolic blood pressure, mean arterial pressure (MAP), infant weight, infant length, apgar score I and apgar score II which obtained p<0.001, r = 0.587, p = 0.011, r = 0.356, p<0.001, r = 0.497, p = 0.005, r = 0.393, p = 0.001, r = 0.448, p = 0.063, r = 0.265 and p = 0.020, r = 0.328. The relationship of sFlt1 with maternal and neonatal outcome found p = 0.984, r = 0.003, p = 0.783, r = 0.040, p = 0.758, r = 0.045, p = 0.471, r = 0.104, p = 0.292, r = 0.152, p = 0.639, r = 0.068 and p = 0.707, r = 0.055. Conclusion:There was relationship between MDA with maternal and neonatal outcomes except for apgar score in the first minute. There was no relationship between sFlt1 with materal and neonatal outcome.